T/CVDA 4-2022 Active Group standards

T/CVDA 4-2022 The technique for detecting neutralizing antibodies against Middle East respiratory syndrome coronavirus in animals

Publish Date: 2022-07-19 Implement Date: 2022-07-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CVDA 4-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Agriculture, forestry

ICS Name: Veterinary medicine

Publish Date: 2022-07-19

Implement Date: 2022-07-25

Pages: 11 pages

Scope

Scope: This standard specifies the experimental operation methods and result determination criteria for the virus neutralization test (VNT) used to quantify neutralizing antibodies in animal serum against the Middle East Respiratory Syndrome (MERS) coronavirus. This standard applies to the qualitative and quantitative detection of neutralizing antibodies against the MERS coronavirus in animal serum; Main technical content: This technical standard applies to the detection of neutralizing antibodies against the MERS virus in animal serum. The purpose is: Conventional MERS virus neutralizing antibody detection requires being conducted in a biosafety level 3 laboratory, which is not convenient for general units to carry out. This project proposes to replace the whole virus with a safer recombinant virus VSVΔG-eGFP-MERS for virus neutralizing antibody detection. This project proposes to stipulate the technical requirements for the detection of neutralizing antibodies against the MERS virus in animals and establish diagnostic standards for the detection of neutralizing antibodies against the MERS virus in animals. Significance: This detection technology greatly reduces the biosafety risks and requirements, and can be detected in a conventional biosafety level 2 laboratory. It improves the detection ability of neutralizing antibodies against the MERS virus in animals, so as to better grasp the epidemic trend of the disease and provide reference for formulating effective prevention and control measures. At the same time, it can provide a basis for evaluating the immunization effect of MERS vaccines in animals. This technical standard applies to the detection of neutralizing antibodies against the MERS virus in animal serum. Through the detection of neutralizing antibodies against the MERS virus in serum, it helps to judge whether animals are infected with the MERS virus or evaluate the immunization effect of MERS vaccines in animals. The main technical content is: 1. Constr