T/CAIA SH017-2021 Industrial cannabis: Determination of content of cannabinoids, tetrahydrocannabinol (THC), and tetrahydrocannabinol acid using high-performance liquid chromatography (HPLC) method
T/CAIA SH017-2021 Industrial cannabis: Determination of content of cannabinoids, tetrahydrocannabinol (THC), and tetrahydrocannabinol acid using high-performance liquid chromatography (HPLC) method
Basic Information
Scope
Scope: This document specifies the principles, instruments and reagents, analytical steps, instrument measurement conditions, result calculation and presentation, and data accuracy for determining the content of cannabinol, tetrahydrocannabinol, and tetrahydrocannabinolic acid in industrial hemp. This document is applicable to the determination of the content of cannabinol, tetrahydrocannabinol, and tetrahydrocannabinolic acid in industrial hemp, with a quantitative measurement range of 0.2 mg/g to 10 mg/g. Main technical content: After drying, crushing, grinding, and sieving industrial hemp samples, they are extracted with 95% ethanol solution, filtered through a microporous membrane, separated by reverse-phase chromatography, and detected by an ultraviolet detector. The quantification is performed using an external standard method.