T/CVDA 1-2022 Active Group standards

T/CVDA 1-2022 Animal neutralizing antibody detection technology for West Nile virus

Publish Date: 2022-07-19 Implement Date: 2022-07-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CVDA 1-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Agriculture, forestry

ICS Name: Veterinary medicine

Publish Date: 2022-07-19

Implement Date: 2022-07-25

Pages: 10 pages

Scope

Scope: This standard specifies the experimental operation methods and result determination criteria for the plaque reduction neutralization test used to measure neutralizing antibodies in animal serum against West Nile virus. This standard is applicable to the qualitative and quantitative detection of neutralizing antibodies against West Nile virus in animal serum. Main technical content: This technical standard is suitable for the detection of neutralizing antibodies against West Nile virus in serum of animals susceptible to the virus. By detecting neutralizing antibodies against West Nile virus in serum, it can assist in determining whether an animal is infected with West Nile virus or evaluating the immunization effect of the West Nile fever vaccine. The main technical content includes: 1. Construction of a cell line stably expressing the West Nile virus C-prM-E protein; 2. Construction of a West Nile virus replicon lacking the cellular protein gene and expressing green fluorescent protein; 3. Packaging and titration of the West Nile virus replicon; 4. Collection and inactivation of animal serum; 5. Neutralization of serum with the West Nile virus replicon and cell inoculation; 6. Observation and determination of results through fluorescent foci; 7. Observation and determination of results through virus plaque count.