T/GXAS 489-2023: Differential Detection of African Swine Fever Virus, Swine Fever Virus, and Swine Atypical Pestivirus by Multiplex RT-PCR and Multiplex qRT-PCR Methods
T/GXAS 489-2023
Basic Information
Scope
Scope: This document specifies the techniques for identifying and detecting African swine fever virus, swine fever virus, and porcine reproductive and respiratory syndrome virus (PRRSV) using multiple RT-PCR and multiple qRT-PCR methods, including principles, experimental conditions, reagents, main instruments and equipment, sample collection and processing, multiple RT-PCR detection, multiple qRT-PCR detection, negative and positive controls, and result interpretation. This document is applicable to the detection of nucleic acids of African swine fever virus, swine fever virus, and porcine reproductive and respiratory syndrome virus (PRRSV). Main technical content: This document specifies the techniques for identifying and detecting African swine fever virus, swine fever virus, and porcine reproductive and respiratory syndrome virus (PRRSV) using multiple RT-PCR and multiple qRT-PCR methods, including principles, experimental conditions, reagents, main instruments and equipment, sample collection and processing, multiple RT-PCR detection, multiple qRT-PCR detection, negative and positive controls, and result interpretation. This document is applicable to the detection of nucleic acids of African swine fever virus, swine fever virus, and porcine reproductive and respiratory syndrome virus (PRRSV).