T/CAS 508-2021 AOC Testing Method Based on Flow Cytometry
T/CAS 508-2021
Basic Information
Scope
Scope: This standard specifies the methodological principles, reagents and materials, instruments and equipment, sampling and preservation, experimental procedures, record-keeping, precision, and quality assurance for the determination of assimilable organic carbon (AOC) in drinking water using flow cytometry. This standard is applicable to the determination of AOC in drinking water. Main technical content: 1. Scope: This standard specifies the methodological principles, reagents and materials, instruments and equipment, sampling and preservation, experimental procedures, record-keeping, precision, and quality assurance for the determination of assimilable organic carbon (AOC) in drinking water using flow cytometry. This standard is applicable to the determination of AOC in drinking water. 2. Normative references: The following documents are essential for the application of this document. For dated reference documents, only the version dated at the time is applicable to this document. For undated reference documents, the latest version (including all amendments) is applicable to this document. - GB/T 39730-2020: General Requirements for Cell Counting - Flow Cytometry Method - YY/T 0588-2017: Flow Cytometer 3. Terms and definitions: The terms and definitions defined in GB/T 39730-2020 and YY/T 0588-2017, as well as the following terms and definitions, are applicable to this document. 3.1 Cell counting: Measuring and calculating the number of target cells in a sample, including cells of any state (dead or alive), type (genotype or phenotype), and function (e.g., activity). 3.2 Flow cytometry (FCM): A modern analytical technique using flow cytometers to measure the physical and chemical properties of suspended cells or particles in a liquid phase, such as cell size, internal structure, DNA, RNA, proteins, antigens, etc., for rapid determination and sorted collection. 3.3 Cell staining: The binding of fluorescein-conjugated specific antibodies or dyes with various cyt