GB/T 26322-2010
Active
T/SDAQI 007-2021
Active
Group standards
T/SDAQI 007-2021 The rapid qualitative detection of Pseudomonas aeruginosa in water production using real-time PCR method
T/SDAQI 007-2021 The rapid qualitative detection of Pseudomonas aeruginosa in water production using real-time PCR method
Price:
$ 20.00
Basic Information
Standard Code:
T/SDAQI 007-2021
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Medicine, health, and labor protection
ICS Name:
The testing of the biological properties of water
Publish Date:
2021-09-25
Implement Date:
2021-10-25
Scope
Scope: This document is applicable to the rapid qualitative detection of Pseudomonas aeruginosa in production water; Main technical content: After the sample is filtered by a vacuum filtration device, the filtered membrane is placed in a selective medium for cultivation. The cultured bacterial solution is collected for DNA extraction. Using DNA as the template, the bacterial 16S rDNA gene sequence is used as an internal reference, and primers and probes that are relatively conserved and highly specific to the exotoxin A (eta) gene in Pseudomonas aeruginosa are used for real-time fluorescence PCR amplification of the target. Based on the Ct value, it is determined whether the sample contains Pseudomonas aeruginosa. Among them, the detection of the internal reference reaction can monitor whether the reaction is normal and prevent false negative results.
Development Information
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