T/NSFST 009-2022 Sunlight Greenhouse Production Techniques for Big Cylindrical Gourmet Mushroom

Scope

Main Technical Content: 3.1 Stropharia rugosoannulata Farl. ex Murrill This species belongs to the Basidiomycota phylum, Agaricales order, and Strophariaceae family. Its aliases include *wrinkled ring Stropharia*, *wine-red Stropharia*, and *Pei's Stropharia*. 3.2 Master Seed A pure culture of mycelium derived from the transplantation and expansion of master seed, also known as second-generation seed. 3.3 Cultivation Seed A pure culture of mycelium derived from the transplantation and expansion of master seed. Cultivation seed can only be used for cultivation and cannot be used for further seed expansion. Also known as third-generation seed. 4. Master Seed Production 4.1 Production Conditions The production site, plant layout, and equipment must comply with the requirements of NY/T 528 and NY/T 2375. 4.2 Selection of Stropharia rugosoannulata Master Seed Master seed should be purchased from qualified units and transported at ≤4°C. The seed should be inoculated as soon as possible after receipt. The variety and quality of the seed source must meet the requirements of NY/T 528. 4.3 Selection of Cultivation Seed Production Time In Ningxia, the suitable sowing time in sunlit greenhouses is from October to April. Cultivation seed should be produced 25–20 days in advance of the sowing time. 4.4 Cultivation Seed Substrate Formulation and Pre-treatment Methods 50% rice husk, 40% corn cob (crushed to 0.5–3 cm in size), 9.0% wheat bran, and 1.0% gypsum. The rice husk and corn cob should be soaked for 12–24 hours, then mixed with wheat bran and gypsum and adjusted to 60–65% water content. The substrate raw materials must meet the requirements of NY/T 1935. 4.5 Bagging of Cultivation Seed Use 14-cm × 28-cm × 0.4-mm polypropylene bags with a filling height of 15.0–16.0 cm. Place a 2-cm × 15-cm inoculation rod in the center, then seal with a 4-cm-diameter cotton-free cap/neck ring. High-pressure sterilize at 121°C for 2.5 hours. Transfer t

Development Information

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