T/GOTA 009-2025 Active Group standards

T/GOTA 009-2025 Quantitative fluorescence resonance energy transfer microimaging assays

Publish Date: 2025-11-13 Implement Date: 2025-11-30 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GOTA 009-2025

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Medical optical instruments and equipment, and endoscopes

ICS Name: Optical measurement instruments

Publish Date: 2025-11-13

Implement Date: 2025-11-30

Scope

This document is applicable to quantitative fluorescence resonance energy transfer microscopy detection, and the specific scenarios include but are not limited to: the interaction between two molecules in living cells; the conformational changes of a single molecule in living cells; and the binding ratio of two molecules in a complex in living cells. This document describes a method for quantitatively detecting biomolecular events in living cells using fluorescence resonance energy transfer microscopy technology, including detection environment requirements, instrument requirements, detection preparation, system calibration, and quantitative FRET detection.