T/SDAA 0046-2021 Active Group standards

T/SDAA 0046-2021: Detection of Clostridium perfringens in animal feces

T/SDAA 0046-2021

Publish Date: 2021-11-30 Implement Date: 2021-12-31 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/SDAA 0046-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: \nLivestock husbandry

ICS Name: Animal husbandry and breeding

Publish Date: 2021-11-30

Implement Date: 2021-12-31

Scope

Main technical content: 3. Principle: Based on the ability of Clostridium perfringens to secrete alpha toxin and theta toxin, which cause hemolysis in blood agar medium, preliminarily screen out suspect bacteria; Based on the characteristics of Clostridium perfringens as a Gram-positive rod-shaped bacterium, the ability of alpha toxin to decompose lecithin in egg yolk, which results in a milky-white hazy ring on egg yolk agar medium, and its most prominent biochemical characteristics: fermenting lactose in milk to cause milk acid coagulation and produce large amounts of gas, leading to the rupture of clots into porous spongy "burst fermentation" phenomenon, preliminarily identify Clostridium perfringens; Based on the 16s rDNA gene sequence of this bacterium, design a pair of specific primers, amplify a specific target gene fragment using DNA as the template, and determine the size of the PCR product to be 220 bp through agarose gel electrophoresis, confirming the identity of Clostridium perfringens.