T/AFFI 014-2022 Determination of Flavonoid Compounds in Red Dates

Scope

Main Technical Content: Determination of Flavonoids in Red Dates 1 Scope This standard applies to red dates from the Xinjiang region. It specifies the testing methods for flavonoids in red dates, including cyclic adenosine monophosphate (cAMP), rutin, quercetin, and chlorogenic acid. This document is applicable to the detection of flavonoids in fresh and dried red dates in this region. 2 Normative References The referenced documents in this document are essential for its application. For dated referenced documents, only the version specified on the date applies to this document. For undated referenced documents, the latest version (including all amendments) applies. GB/T 6682: Specifications and Test Methods for Water Used in Analytical Laboratories 3 Terms and Definitions 3.1 Fresh Red Dates Fresh, clean red dates that have undergone certain processing or preservation treatments. 3.2 Dried Red Dates Red dates processed by natural drying or baking. 3.3 Flavonoids Originally, this term referred to a class of compounds derived from 2-phenylchromone. Now, it broadly refers to a series of compounds with a C6-C3-C6 structure. 4 Principle After extraction of cAMP, rutin, quercetin, and chlorogenic acid from red dates with water and 30% methanol-water solution, they are separated by an ODS-BP chromatographic column and detected at wavelengths of 254 nm and 325 nm using a diode array detector. Quantification is performed using external standards. 5 Reagents and Materials Unless otherwise specified, only reagents recognized as analytical grade and first-class water specified in GB/T 6682 are used in the analysis. 5.1 Reagents Potassium dihydrogen phosphate, methanol (ultra-pure grade). 5.2 Standard Samples cAMP standard, rutin, quercetin, and chlorogenic acid standard samples. 5.3 Standard Solution Preparation Preparation of standard stock solution (1 mg/mL): Accurately weigh 0.0001 g of each control sample an

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