T/DEFA 003-2025 Black mushroom residue cultivation for mantou technology specification

Scope

7.1 Medium Preparation 7.1.1 Cultivation Substrate The main ingredients of the cultivation substrate include black fungus mycelium residue, broad-leaved hardwood sawdust, corn cobs, and other auxiliary materials such as rice chaff and bran, which should comply with the requirements of NY 5099. 7.1.2 Reference Formulas Formula 1: Black fungus mycelium residue 50%, sawdust 30%, rice chaff (bran) 18%, gypsum powder 1%, and lime powder 1%; Formula 2: Black fungus mycelium residue 40%, corn cobs 40%, rice chaff (bran) 18%, gypsum powder 1%, and lime powder 1%; Formula 3: Black fungus mycelium residue 30%, soybean straw 25%, corn cobs 25%, rice chaff (bran) 18%, gypsum powder 1%, and lime powder 1%; All the formulas have a water content of 55% to 58% and a natural pH. 7.2 Mycelium Bag Production 7.2.1 Raw Material Processing Treatment of mycelium residue: Select less contaminated black fungus mycelium residue. After mechanical separation, grind and dry the mycelium residue; Treatment of corn cobs: Crush into particles with a size of 0.5 cm to 1.0 cm; Treatment of soybean straw: Crush into small segments with a size of 1.0 cm to 1.5 cm. 7.2.2 Pre-wetting and Softening of Raw Materials Add appropriate water (or a 1% lime solution) to soak and soften the sawdust, corn cobs, and soybean straw for 12 to 24 hours in advance. 7.2.3 Mixing and Bagging After sieving, add the raw and auxiliary materials together into the mixing machine and stir while adding water to achieve a water content of 55% to 60% in the substrate. Mix evenly. Use (16.0 cm to 16.5 cm) × (35.0 cm to 38.0 cm) polyethylene or polypropylene plastic bags for mechanical bagging, with a suitable tightness of the bag and a height of 22 cm. Each bag should contain 1.2 kg to 1.3 kg of material. 7.2.4 Sterilization For autoclave sterilization, maintain a temperature of 100 °C for 7 to 8 hours after ignition, and then incubate for 3 to 5 hours after shutting off the fire. For high

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