T/CDZX 003-2023 Determination of Phospholipidoylcholine in Milk Powder by High Performance Liquid Chromatography (HPLC) Method
T/CDZX 003-2023 Determination of Phospholipidoylcholine in Milk Powder by High Performance Liquid Chromatography (HPLC) Method
Basic Information
Scope
Scope: This document specifies a high-performance liquid chromatography method for the determination of phosphatidylcholine in milk powder. This document is applicable to the determination of phosphatidylcholine in milk powder. When the sampling amount is 1 g and the volume is adjusted to 5.0 mL, the detection limit is 5 mg/100 g, and the quantification limit is 10 mg/100 g; Main technical content: According to the principle of "like dissolves like", the chloroform-methanol solution (2+1, V/V) can dissolve and extract phosphatidylcholine well. After extraction, the solution is filtered through an organic filter membrane, and the phosphatidylcholine content is determined by high-performance liquid chromatography using an external standard method for quantification.