T/CAIA SH005-2015 Determination of Furanazone Metabolite 3-Amino-2-Oxazolidinone in Animal-derived Food by Enzyme-Linked Immunosorbent Assay (ELISA)

Scope

Scope: This standard specifies the enzyme-linked immunosorbent assay (ELISA) method for the determination of furazolidone metabolite 3-amino-2-oxalidinone (AOZ) in animal-derived foods. This standard is applicable to the determination of furazolidone metabolite 3-amino-2-oxalidinone in animal-derived foods such as chicken, chicken liver, pork, pork liver, fish, and shrimp. Other foods can be tested in accordance with this standard. The method's detection limit is 0.10 μg/kg. Main technical content: This method is an indirect competitive ELISA method. 2-Nitrobenzaldehyde reacts with 3-amino-2-oxalidinone in the sample to form 3-(2-nitrobenzylideneamino)-2-oxazolidinone (2-NP-AOZ). The microwells of the ELISA plate are coated with 2-NP-AOZ antigen. The antigen specifically competes with the 2-NP-AOZ antigen in the sample for the 2-NP-AOZ antibody. The enzyme-labeled secondary antibody is added, and the color is developed with a colorimetric solution. The content of 2-NP-AOZ in the sample is inversely proportional to the relative absorbance value. The absorbance is measured at a wavelength of 450 nm using an ELISA reader, and the content of furazolidone metabolite in the sample is calculated with analysis software.

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