T/CSTM 00956-2024 Active Group standards

T/CSTM 00956-2024 Spherical Protein Apparent Molecular Weight Detection by Liquid Nuclear Magnetic Resonance Spectroscopy

T/CSTM 00956-2024

Publish Date: 2024-10-31 Implement Date: 2025-01-31 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CSTM 00956-2024

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Integrated

ICS Name: Terminology (principles and coordination and cooperation)

Publish Date: 2024-10-31

Implement Date: 2025-01-31

Scope

Scope: This document specifies the terms and definitions, principles, reagents and materials, instrumentation, samples, experimental procedures, experimental data processing, and precision for determining the apparent molecular weight of spherical proteins using liquid NMR spectrometers. This document is applicable to the determination of the apparent molecular weight of spherical soluble proteins, including proteins from tissue extraction, recombinant expression, and biological preparations, using the diffusion-ordered spectroscopy (DOSY) experiment of liquid NMR spectrometers. The molecular weight of the proteins is generally less than 50 kDa, with a concentration range of 0.05 mmol/L to 1.00 mmol/L. The protein solution may contain additives such as buffer solutions and salts. Main technical content: This document specifies the terms and definitions, principles, reagents and materials, instrumentation, samples, experimental procedures, experimental data processing, and precision for determining the apparent molecular weight of spherical proteins using liquid NMR spectrometers. This document is applicable to the determination of the apparent molecular weight of spherical soluble proteins, including proteins from tissue extraction, recombinant expression, and biological preparations, using the diffusion-ordered spectroscopy (DOSY) experiment of liquid NMR spectrometers. The molecular weight of the proteins is generally less than 50 kDa, with a concentration range of 0.05 mmol/L to 1.00 mmol/L. The protein solution may contain additives such as buffer solutions and salts.