T/GZCX 018-2022 Active Group standards

T/GZCX 018-2022 Determination of total flavonoids content in Ribes nigrum L. and its products

Publish Date: 2022-11-22 Implement Date: 2022-12-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GZCX 018-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Fruits and their products

Publish Date: 2022-11-22

Implement Date: 2022-12-01

Scope

Scope: This standard specifies the ultraviolet spectrophotometric method for determining the total flavonoid content (expressed as rutin) in prickly pear and its products. This standard is applicable to the determination of total flavonoid content in fresh prickly pears, dried prickly pears, prickly pear preserves, freeze-dried prickly pear powder, spray-dried prickly pear powder, prickly pear juice, and concentrated prickly pear juice, among other prickly pear and its products; Main technical content: 4. Method principle: Flavonoids are a class of natural compounds with a benzopyran ring structure. Flavonoids soluble in ethanol react with aluminum salts under weak alkaline conditions to form colored substances, which exhibit maximum absorption at around 420 nm. Within a certain concentration range, the absorbance is directly proportional to the content of flavonoids. By comparing with the rutin standard, the content of flavonoids can be quantitatively determined. 5. Reagents and materials 5.1 Reagents: Except where otherwise specified, only analytical-grade reagents and Class I water specified in GB/T 6682 are used. 5.1.1 Rutin (C27H30O16, CAS No.: 153-18-4) standard sample, with a purity of ≥ 98.0%. 5.1.2 Anhydrous ethanol (C2H6O). 5.1.3 Aluminum nitrate [Al.(NO3)3·9H2O]. 5.1.4 Potassium acetate (C2H3KO2). 5.2 Preparation of reagents 5.2.1 Rutin control solution (1 mg/mL): Precisely weigh 50 mg (with an accuracy of 0.1 mg) of rutin standard sample, dissolve it in a small amount of anhydrous ethanol, transfer to a 50 mL volumetric flask, and make up to the mark with anhydrous ethanol. Shake well. 5.2.2 Aluminum nitrate solution (100 g/L): Weigh 17.60 g (with an accuracy of 0.01 g) of Al(NO3)3·9H2O, dissolve it in a small amount of water, transfer to a 100 mL volumetric flask, and make up to the mark with water. Shake well. 5.2.3 Potassium acetate solution (98 g/L): Weigh 9.80 g (with an accuracy of 0.01 g) of C2H3KO2, dissolve it in a small am