T/NXFSA 001-2019 Quick detection method for 12 pesticide residues in Goji berries: Colloidal gold immunochromatography method

Scope

Scope: This standard specifies a rapid detection method using colloidal gold immunoassay for the residues of dinotefuran, imidacloprid, chlorpyrifos, multisporin, cypermethrin, chlorpyrifos-methyl, cyhalothrin, cypermethrin, fenpyroximate, carbofuran, acaricidin, methamidophos, and azoxystrobin in fresh and dried wolfberry fruits. This standard applies to the rapid detection of 12 pesticide residues (dinotefuran, imidacloprid, chlorpyrifos, multisporin, cypermethrin, chlorpyrifos-methyl, cyhalothrin, cypermethrin, fenpyroximate, carbofuran, acaricidin, methamidophos, and azoxystrobin) in fresh and dried wolfberry fruits. Main technical content: A rapid detection method using colloidal gold immunoassay for 12 pesticide residues in wolfberry fruits. 2. Principle: Based on the principle of colloidal gold competitive inhibition immunoassay, the residues of the target substances in the sample compete with the specific antibody labeled with colloidal gold on the detection line. By comparing the color intensity of the detection line and the control line, the qualitative and semi-quantitative determination of the target substances in the sample can be carried out. 3. Reagents and materials 3.1 Colloidal gold immunoassay kit: A colloidal gold immunoassay kit for pesticide residues, including colloidal gold test strips and accompanying reagents. 3.2 Extraction solution 3.2.1 Water-soluble pesticide extraction solution: Dinotefuran, imidacloprid, chlorpyrifos, multisporin, carbofuran, acaricidin, and methamidophos. Use pure water or distilled water as the extraction solution. 3.2.2 Non-soluble or difficult-to-water pesticide extraction solution: Cyhalothrin, chlorpyrifos-methyl, cypermethrin, and fenpyroximate. Use a 1:1 mixture of water and anhydrous ethanol (analytical grade) as the extraction solution. 4. Instrumentation and equipment 4.1 Electronic balance (with a sensitivity of 0.1g). 4.2 Incubator: 40℃ ± 2℃ (optional). 4.3 Portable reader. Measurement wavelength

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