T/GXAS 492-2023 Determination of water quality using high-performance liquid chromatography-tandem mass spectrometry for the determination of two types of lincosamide and four types of macrolide antibiotics

Scope

Scope: This document describes the method of determining two lincomycin antibiotics and four macrolide antibiotics in water using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). This document is applicable to the determination of lincomycin, clindamycin, oleandomycin, tylosin, leucomycin A5, and josamycin (a total of two lincomycin antibiotics and four macrolide antibiotics) in surface water and domestic wastewater. Other lincomycin and macrolide compounds can be measured after suitability verification. When the injection volume is 5.0 μL, the detection limit of the direct injection method is 1.1 μg/L to 1.6 μg/L, and the lower limit of determination is 4.4 μg/L to 6.4 μg/L. When the enriched sample volume is 200 mL, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the detection limit of the solid-phase extraction method is 2.4 ng/L to 9.7 ng/L, and the lower limit of determination is 9.6 ng/L to 38.8 ng/L. For details, please refer to Appendix A. Main technical content: This document describes the method of determining two lincomycin antibiotics and four macrolide antibiotics in water using HPLC-MS/MS. This document is applicable to the determination of lincomycin, clindamycin, oleandomycin, tylosin, leucomycin A5, and josamycin (a total of two lincomycin antibiotics and four macrolide antibiotics) in surface water and domestic wastewater. Other lincomycin and macrolide compounds can be measured after suitability verification. When the injection volume is 5.0 μL, the detection limit of the direct injection method is 1.1 μg/L to 1.6 μg/L, and the lower limit of determination is 4.4 μg/L to 6.4 μg/L. When the enriched sample volume is 200 mL, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the detection limit of the solid-phase extraction method is 2.4 ng/L to 9.7 ng/L, and the lower limit of determination is 9.6 ng/L to 38.8 ng/L. For details, please refer to A

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