GB/T 12715-1991
Abolished
T/LTIA 18-2022
Active
Group standards
T/LTIA 18-2022 Spatial Transcriptomics Operating Guidelines for Preparing Spatial Transcriptome Samples
T/LTIA 18-2022
Price:
$ 20.00
Basic Information
Standard Code:
T/LTIA 18-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
-
ICS Name:
Biology, botany, and zoology
Publish Date:
2022-09-20
Implement Date:
2022-09-30
Scope
Scope: This document provides preparation recommendations for frozen and paraffin-embedded tissue samples for spatiotemporal transcriptomics research. This document is applicable to institutions conducting spatiotemporal genomics research. Main technical content: For paraffin-embedding, follow the following steps: a) Pour melted paraffin (hard wax) into the embedding mold; b) Place the wax-immersed tissue blocks face down on the cut surface or specified side and embed them in the mold containing molten paraffin; c) Place the tissue blocks flat in the center of the bottom of the embedding mold to avoid the formation of air bubbles; d) When the wax block cools to the point where a transparent wax membrane appears on the wax surface, immerse it in cold water to rapidly cool it; e) Remove the embedded tissue blocks from the embedding mold, label the samples on the wax blocks to avoid confusion, and f) Use a blade to trim them into regular squares or rectangles, allowing the cut surface of the embedded tissue blocks to be visually observed. For sectioning: a) Use the microtome according to the manufacturer's instructions. The thickness of the tissue sections is specified in Appendix B; b) Ensure that the running direction of the microtome blade is parallel to the cut surface of the embedded tissue blocks, and adjust the required section thickness according to the sample type and intended requirements before sectioning. c) Check whether the sections are intact, uniform, free from knife marks or tremors, and free from wrinkles, cracks, or defects. d) Place suitable paraffin tissue sections in warm water at 37–50°C to unravel them and attach them to the slides. Avoid the formation of air bubbles between the sections and the slides during the attachment process. e) Tilt the slides to drain off excess water and bake them. f) Attach sample barcode labels to one end of the slides and conduct tissue imaging after HE staining.