GB/T 18415-2001
Active
T/CAIA SH009-2017
Active
Group standards
T/CAIA SH009-2017 Salmonella in food real-time fluorescence nucleic acid constant temperature amplification
T/CAIA SH009-2017 Salmonella in food real-time fluorescence nucleic acid constant temperature amplification
Price:
$ 20.00
Basic Information
Standard Code:
T/CAIA SH009-2017
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Health
ICS Name:
General methods for food testing and analysis
Publish Date:
2017-07-20
Implement Date:
2017-10-01
Scope
Scope: This standard specifies the SAT testing method for Salmonella spp. in food. This standard is applicable to the detection of Salmonella in meat, eggs, dairy products, and processed foods, etc. Main technical content: Under the same temperature, using bacterial RNA as the starting template, the target RNA is converted into a double-stranded DNA by M-MLV reverse transcriptase, and then multiple (100-1000) RNA copies are produced using T7 RNA polymerase as the template for this DNA; each RNA copy then enters the next amplification cycle from the reverse transcription; simultaneously, the optimized probes with fluorescent markers specifically bind to these RNA copies, opening the stem-loop structure and generating fluorescence. Based on the appearance time and intensity of the real-time fluorescence signal, combined with positive and negative controls, the detection results can be determined.
Development Information
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