T/CCAA 42-2022 The qualitative detection method for coix seeding-derived components in mixed grain products using real-time fluorescence PCR
T/CCAA 42-2022 The qualitative detection method for coix seeding-derived components in mixed grain products using real-time fluorescence PCR
Basic Information
Scope
Scope: This document describes the method for qualitatively detecting coix-derived components in mixed grain products using real-time fluorescent PCR. This document is applicable to the qualitative detection of coix-derived components in mixed grain products, including Chinese cultivated coix. The detection limit (LOD) specified in this document is 0.01% (by mass ratio). Main technical content: Foreword 1 Scope 2 Normative reference documents 3 Terms, definitions, and abbreviations 3.1 Terms and definitions 3.2 Abbreviations 4 Principle 5 Primers and probes for detection 6 Reagents and materials 7 Instruments and equipment 8 Detection steps 8.1 DNA extraction 8.2 Determination of DNA concentration and purity 8.3 Real-time fluorescent PCR detection 9 Quality control 10 Result determination and expression 11 Result reporting 12 Anti-contamination measures Appendix A (Normative) Reagents and their preparation Appendix B (Informative) Reference sequences of gene amplification targets for coix-derived components