T/GXAS 494-2023 Water quality determination of seven penicillins using high performance liquid chromatography-tandem mass spectrometry

Scope

Scope: This document describes a method for determining seven types of penicillins in water using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). This document is applicable to the determination of Penicillin G, Meticillin, Azlocillin, Piperacillin, Penicillin V, Cloxacillin, and Dicloxacillin in surface water and domestic wastewater. Other penicillin compounds can be tested after suitability verification. When the injection volume is 5.0 μL, the detection limit of the direct injection method is 1.1 μg/L to 1.7 μg/L, and the quantification limit is 4.4 μg/L to 6.8 μg/L. When the enriched sample volume is 200 mL and the concentrated and diluted volume is 1.0 mL, the detection limit of the solid-phase extraction (SPE) method is 2.7 ng/L to 11.0 ng/L, and the quantification limit is 10.8 ng/L to 44.0 ng/L. For details, please refer to Appendix A. Main Technical Content: This document describes a method for determining seven types of penicillins in water using HPLC-MS/MS. This document is applicable to the determination of Penicillin G, Meticillin, Azlocillin, Piperacillin, Penicillin V, Cloxacillin, and Dicloxacillin in surface water and domestic wastewater. Other penicillin compounds can be tested after suitability verification. When the injection volume is 5.0 μL, the detection limit of the direct injection method is 1.1 μg/L to 1.7 μg/L, and the quantification limit is 4.4 μg/L to 6.8 μg/L. When the enriched sample volume is 200 mL and the concentrated and diluted volume is 1.0 mL, the detection limit of the SPE method is 2.7 ng/L to 11.0 ng/L, and the quantification limit is 10.8 ng/L to 44.0 ng/L. For details, please refer to Appendix A.

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