T/GBC 92-2025 Quick identification of authenticity of anisi stellati fructus—Real-time PCR method
T/GBC 92-2025 Quick identification of authenticity of anisi stellati fructus—Real-time PCR method
Basic Information
Scope
This document defines the terms and definitions of the real-time fluorescent PCR method for rapid identification of the authenticity of star anise, and specifies the abbreviations, principles, reagents and materials, instruments and equipment, sample preparation, testing steps, quality control measures, result determination and expression, and measures to prevent cross-contamination during the detection process of the authenticity of star anise. This document is applicable to the real-time fluorescent PCR identification of the authenticity of star anise raw fruits, crushed bodies, and powders. Fresh star anise fruits can be used as a reference for implementation. The detection limit (LOD) is 5% (by mass fraction). This document is applicable to the real-time fluorescent PCR identification of the authenticity of star anise raw fruits, crushed bodies, and powders. Fresh star anise fruits can be used as a reference for implementation.