GB/T 12715-1991
Abolished
T/LTIA 24-2023
Active
Group standards
T/LTIA 24-2023 Digitalization of Genetic Information for White Rose Germplasm Resources Technical Specification
T/LTIA 24-2023 Digitalization of Genetic Information for White Rose Germplasm Resources Technical Specification
Price:
$ 20.00
Basic Information
Standard Code:
T/LTIA 24-2023
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
-
ICS Name:
Biology, botany, and zoology
Publish Date:
2023-12-06
Implement Date:
2023-12-07
Scope
Scope: This document specifies the requirements for the collection and processing of Agathis odorata samples, the preparation and quality control of Agathis odorata nucleic acid samples, the collection and acquisition of Agathis odorata genetic information, and the construction and management of the Agathis odorata genetic information database. This document is applicable to the digitization of Agathis odorata germplasm resources. Main technical content: In terms of terminology and definitions, the terms "germplasm" and "germplasm resources" are defined. In the collection and processing of Agathis odorata samples, based on field research practices and practical work experience, the collection and processing procedures for Agathis odorata leaf samples and root samples are standardized. In the preparation and quality control of Agathis odorata nucleic acid samples, referring to GB/T 40664 "General Requirements for Quality Control of Nucleic Acid Samples Used for High-Throughput Sequencing" and commercial nucleic acid extraction kits, combined with practical nucleic acid extraction operation experience and scientific literature, the preparation and quality control requirements for Agathis odorata nucleic acid samples (genomic DNA, total RNA) are standardized. In the collection of Agathis odorata genomic information and the construction of the genomic database, based on standards such as GB/T 30989 "High-Throughput Gene Sequencing Technology Specifications" and GB/T 35890 "High-Throughput Sequencing Data Sequence Format Specifications", as well as practical work needs, the metadata and sequencing information storage requirements for Agathis odorata genomic DNA and total RNA sequencing and the genomic database are standardized.