GB/T 12715-1991
Abolished
T/CSCB 0005-2022
Abolished
Group standards
T/CSCB 0005-2022 Human intestinal organoids
T/CSCB 0005-2022 Human intestinal organoids
Price:
$ 20.00
Basic Information
Standard Code:
T/CSCB 0005-2022
Standard Type:
Group standards
Standard Status:
Abolished
is_force_gb:
no
CCS Name:
Integrated
ICS Name:
Biology, botany, and zoology
Publish Date:
2022-08-30
Implement Date:
2022-10-31
Scope
Scope: This document specifies the ethical requirements, technical requirements, and detection methods for human intestinal organoids. This document applies to the in vitro preparation and detection of organoids derived from human intestinal epithelial tissues; Main technical content: Foreword 1 ; Scope 2; Normative reference documents 3; Terms and definitions 4; Abbreviations 5; Ethical requirements 6; Technical requirements 7; Detection methods; Appendix A (Normative) Cell type marker genes Real-time fluorescence quantitative PCR method; Appendix B (Normative) Detection of the proportion of various cell types Immunofluorescence staining method; Appendix C (Normative) Alkaline phosphatase detection Alkaline phosphatase staining method; Appendix D (Normative) Mucin detection Mucin staining method; Appendix E (Normative) Organoid survival rate detection Calcein staining method; Appendix F (Normative) STR identification of organoids; References.