T/CIS 67003-2023 Detection of four fish-borne toxins in the rabbit head thunnus species and its processed foods using real-time fluorescence PCR

Scope

Scope: This document describes the principles, detection primers and probes, reagents and preparation, instruments and equipment, detection steps, quality control, result determination and expression, detection limits, and measures to prevent cross-contamination during the detection process for the determination of four pufferfish species (Lagocephalus inermis, Lagocephalus lunaris, Lagocephalus spadiceus, and Lagocephalus gloveri) in fish and their processed products (such as fish fillets, fish balls, fish meal, fish paste, etc.). Other foods and feeds containing fish ingredients can be used for reference. Main technical content: Table of Contents Foreword II. Introduction III. 1. Scope 2. Normative references 3. Terms and definitions 4. Abbreviations 5. Principles 6. Detection primers and probes 7. Reagents and preparation 7.1. Reagents 7.2. Solution preparation 8. Instruments and equipment 9. Detection steps 9.1. Sampling and sample preparation 9.2. Genomic DNA extraction 9.3. Determination of genomic DNA concentration and purity 9.4. PCR detection 10. Quality control 11. Result determination and expression 11.1. Result determination 11.2. Result expression 12. Detection limits 13. Measures to prevent cross-contamination during the detection process 14. Appendix A (informative) 15. Reference sequences of the gene amplification targets of four pufferfish species 16. References

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