T/GZCX 020-2022 Active Group standards

T/GZCX 020-2022 Determination of Total Phenolic Content in Shrubby Ratibiah and Its Products

Publish Date: 2022-11-22 Implement Date: 2022-12-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GZCX 020-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Fruits and their products

Publish Date: 2022-11-22

Implement Date: 2022-12-01

Scope

Scope: This standard specifies the ultraviolet spectrophotometric method for determining the total phenol content in prickly pear and its products. This standard is applicable to the determination of total phenol content in fresh prickly pear, dried prickly pear, prickly pear preserved fruits, freeze-dried prickly pear powder, spray-dried prickly pear powder, prickly pear juice, and concentrated prickly pear juice, among other prickly pear and its products; Main technical content: 4. Method principle: Under alkaline conditions, polyphenols can reduce phosphotungstic acid and molybdic acid to blue, and the blue intensity is directly proportional to the polyphenol content. The absorbance value is measured at 760 nm, and the quantification is performed by external standard method. 5. Reagents 5.1. Reagents: Except where otherwise specified, only analytical-grade reagents and Grade I water specified in GB/T 6682 are used. 5.1.1. Gallic acid (C7H6O5, CAS: 149-91-7) standard: Purity ≥ 98%. 5.1.2. Anhydrous ethanol (C2H6O). 5.1.3. Sodium carbonate (Na2CO3). 5.1.4. Folin-Phenol reagent (BR, 1 mol/L). 5.2. Preparation of reagents 5.2.1. Gallic acid control solution (0.1 mg/mL): Precisely weigh 10 mg (accurate to 0.1 mg) of gallic acid standard and dissolve it in a small amount of water. Transfer to a 100 mL brown volumetric flask, add water to the mark, shake well, and store at 4 °C in the dark. 5.2.2. Gallic acid standard series concentration solutions: Accurately pipette 0.0 mL, 0.25 mL, 0.50 mL, 0.75 mL, 1.00 mL, 1.25 mL, and 1.5 mL of gallic acid control solution (5.2.1) into a 25 mL brown volumetric flask, and add water to the mark. Shake well and store at 4 °C. The gallic acid standard series concentration is 0μg/mL, 1.0μg/mL, 2.0μg/mL, 3.0μg/mL, 4.0μg/mL, 5.0μg/mL, and 6.0μg/mL. Prepare freshly for use. 5.2.3. 12% sodium carbonate solution: Weigh 12 g of anhydrous sodium carbonate (accurate to 0.01 g), dissolve it in a suitable amount of water, a