GB/T 12715-1991
Abolished
T/LTIA 08-2020
Active
Group standards
T/LTIA 08-2020 SARS-CoV-2 nucleic acid qRT-PCR method detection technical specification
T/LTIA 08-2020 SARS-CoV-2 nucleic acid qRT-PCR method detection technical specification
Price:
$ 20.00
Basic Information
Standard Code:
T/LTIA 08-2020
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
\nLivestock husbandry
ICS Name:
Biology, botany, and zoology
Publish Date:
2020-06-08
Implement Date:
2020-07-08
Scope
Scope: This standard specifies the terms, definitions, and abbreviations of the general qRT-PCR method (Taqman probe method) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the detection principle, pre-experiment preparation, sample collection, storage and transportation, detection process, and requirements for result determination. This standard applies to institutions conducting qRT-PCR detection of SARS-CoV-2 by detecting the nucleic acid of suspected infected patients. Main technical content: The qRT-PCR detection of SARS-CoV-2 nucleic acid uses a method combining PCR amplification and fluorescent probes. Specific primers and fluorescent dual-labeled probes are synthesized targeting the highly conserved sequence of SARS-CoV-2. The probe is labeled with a fluorescent group at the 5' end and a quenching group at the 3' end. During PCR amplification, the specific primers and probes bind to the target sequence. Through the DNA polymerase activity and 5'-3' exonuclease activity of Taq enzyme, the fluorescent probe is cut when it is extended. At this time, the fluorescent group and the quenching group separate, and the quenching effect disappears, generating a fluorescent signal. By detecting the fluorescent signal, the qualitative detection of SARS-CoV-2 nucleic acid in the sample is achieved.