T/SLTSA 019-2016 Phosteria entomophila Harz Field synchronous bee propagation technology regulations

Scope

Scope: This standard specifies the synchronous mass-rearing technology of Aphis gossypii parasitoid wasps in the field. This standard is applicable to the management of the synchronous mass-rearing technology of Aphis gossypii parasitoid wasps in the field by all tobacco technical service cooperatives in Shilin. Main technical content: 1. Construction of field mass-rearing facilities: The location of the mass-rearing shed should be selected in the upwind direction to facilitate the migration of parasitoid wasps. The mass-rearing shed is composed of a frame and a 60-mesh insect-proof net. The insect-proof net is opened at both or one end and controlled by zippers. The surrounding area is compacted to prevent the invasion of Aphis gossypii natural enemies and the escape of Aphis gossypii and parasitoid wasps from the shed during mass-rearing. The shed is covered with shading nets. 2. Cultivation and management of mass-rearing tobacco plants: First, the land is dug and leveled, then the soil is loosened and ridges are formed. The ditch depth should be ≥15cm, and the ridge direction should be conducive to management. The shed is built about 15 days after tobacco planting, and the distance between the tobacco plants and the shed edge should be ≥25cm. Strengthen the water and fertilizer management of tobacco plants to maintain a lush green leaf color. 3. Time of Aphis gossypii breeding and inoculation: When the tobacco plants reach 6-8 effective leaves (the period of cluster formation), Aphis gossypii is inoculated. Selection of aphids: III-aged and IV-aged unparasitized aphids are selected. Inoculation quantity: More than 30 Aphis gossypii per tobacco plant are inoculated. 4. Management of mass-rearing parasitoid wasps: The temperature in the mass-rearing shed should be maintained between 17°C and 27°C, and the humidity between 50% and 80%. Strengthen the water and fertilizer management of tobacco plants to keep the potting soil moist. Remove other insects in the shed. R

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