T/TDSTIA 026-2022 Active Group standards

T/TDSTIA 026-2022 The determination of α-lactalbumin and β-lactoglobulin in infant formula milk powder using gel permeation chromatography

Publish Date: 2022-11-21 Implement Date: 2022-12-20 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/TDSTIA 026-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Other dairy products

Publish Date: 2022-11-21

Implement Date: 2022-12-20

Scope

Main technical content: This standard is formulated for the first time. The methodological principle is to adjust the pH of the sample solution of infant and toddler formula milk powder to 4.60, so that the casein and denatured whey protein will precipitate, while the un-denatured α-lactalbumin and β-lactoglobulin will still remain in the solution. Hydrochloric guanidine is added to the solution, with hydrochloric guanidine as the denaturing agent and β-mercaptoethanol as the reducing agent, breaking the S-S chemical bonds in the protein to form the unfolded monomeric structures of α-lactalbumin and β-lactoglobulin. After separation by gel chromatography column and UV detection, quantification is carried out by external standard method