GB/T 12715-1991
Abolished
T/SHSYCXH 12-2022
Active
Group standards
T/SHSYCXH 12-2022 Human-derived lung organoids cultivation technical specifications
T/SHSYCXH 12-2022 Human-derived lung organoids cultivation technical specifications
Price:
$ 20.00
Basic Information
Standard Code:
T/SHSYCXH 12-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Integrated
ICS Name:
Biology, botany, and zoology
Publish Date:
2022-09-20
Implement Date:
2022-09-20
Scope
Scope: This document specifies the ethical requirements, equipment and reagents, operating procedures, technical requirements, and detection methods for the cultivation of human lung cancer organoids. This document is applicable to the cultivation of human lung cancer organoids for medical and research purposes. Main technical content: 1. Scope 2. Normative reference documents 3. Terms and definitions 4. Abbreviations 5. Ethical requirements 6. Equipment and reagents 7. Operating procedures 8. Technical requirements 9. Detection methods Appendix A (Normative) - Cell count and survival rate detection (Tetrazolium Blue staining method) Appendix B (Informative) - Typical morphological examples of successfully cultured human lung cancer organoids Appendix C (Normative) - Morphological feature detection (Hematoxylin-Eosin staining method) Appendix D (Informative) - Morphological feature examples (Hematoxylin-Eosin staining method) Appendix E (Normative) - Pathological marker expression detection (Immunohistochemistry and immunofluorescence staining methods) Appendix F (Informative) - Pathological marker expression examples (Immunofluorescence staining method) Appendix G (Informative) - Gene mutation detection (High-throughput gene sequencing)