T/SHSYCXH 8-2022 Detection of peripheral blood natural killer cells and natural killer T cell subsets using flow cytometry
T/SHSYCXH 8-2022 Detection of peripheral blood natural killer cells and natural killer T cell subsets using flow cytometry
Basic Information
Scope
Scope: This document describes a method for detecting the proportion of natural killer (NK) cells and natural killer T (NKT) cell subpopulations in human peripheral blood using flow cytometry, including the detection principle, reagents and consumables, instrumentation, pre-processing and analysis parameter settings, analysis steps, result calculation, and method characteristics, etc. This document is applicable to the identification and proportion determination of NK cells and NKT cell subpopulations in human peripheral blood. Main technical content: 1. Scope 2. Normative reference documents 3. Terms and definitions 4. Abbreviations 5. Detection principle 6. Reagents and consumables 7. Instrumentation 8. Pre-processing and analysis parameter settings 9. Analysis steps 10. Result calculation 11. Method characteristics Appendix A (informative) - Schematic diagram of sample detection and analysis Appendix B (informative) - Example of minimum sampling quantity Appendix C (informative) - Example of repeatability