GB/T 12715-1991
Abolished
T/SZGIA 3-2018
Active
Group standards
T/SZGIA 3-2018 Preimplantation Genetic Screening (PGS) High-throughput Sequencing (HTS) Technical Specification for Genetic Analysis of Embryos
T/SZGIA 3-2018 Preimplantation Genetic Screening (PGS) High-throughput Sequencing (HTS) Technical Specification for Genetic Analysis of Embryos
Price:
$ 20.00
Basic Information
Standard Code:
T/SZGIA 3-2018
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Basic disciplines
ICS Name:
Biology, botany, and zoology
Publish Date:
2018-12-07
Implement Date:
2018-12-08
Scope
Scope: This standard specifies the technical requirements for each stage of pre-implantation embryo genetic high-throughput sequencing detection technology, including pre-detection, detection, and post-detection. This standard applies to institutions that conduct high-throughput gene sequencing detection on human embryo samples before implantation, and is used to preliminarily evaluate the results of pre-implantation embryo genetic screening based on human samples. Main technical content: The quality control of whole-genome amplified DNA products mainly includes two aspects: concentration detection and electrophoresis detection. After DNA products are detected by Qubit, the concentration must be no less than 30 ng/μL, with a total amount of more than 1 μg, before proceeding with sequencing analysis. The electrophoresis band range of DNA products must be above 250 bp and not exceed 2000 bp, with the main band at 750 bp. After passing the quality control test, the samples enter the library construction stage. The initial amount for library construction is 300 ng. Currently, the NEB double-stranded DNA fragmentation enzyme is used to fragment the DNA into main bands of 150-200 bp small fragments. Subsequently, the DNA is purified, and after end repair, adapter ligation, PCR amplification, and purification, the library is completed. After passing the BMG quantitative test, the library is sequenced on the instrument.