GB/T 12715-1991
Abolished
T/CSCB 0013-2022
Active
Group standards
T/CSCB 0013-2022 Human gut organoids。
T/CSCB 0013-2022 Human gut organoids。
Price:
$ 20.00
Basic Information
Standard Code:
T/CSCB 0013-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Integrated
ICS Name:
Biology, botany, and zoology
Publish Date:
2022-08-30
Implement Date:
2022-10-31
Scope
Scope: This document specifies the ethical requirements, technical requirements, and testing methods for human intestinal organoids. This document is applicable to the in vitro preparation and testing of organoids derived from human intestinal epithelial tissues. Main technical content: Foreword 1 Scope 2 Normative reference documents 3 Terms and definitions 4 Abbreviations 5 Ethical requirements 6 Technical requirements 7 Testing methods Appendix A (Normative) Cell type marker genes Real-time fluorescence quantitative PCR method Appendix B (Normative) Detection of the proportion of various cell types Immunofluorescence staining method Appendix C (Normative) Alkaline phosphatase detection Alkaline phosphatase colorimetric method Appendix D (Normative) Mucin detection Mucin staining method Appendix E (Normative) Survival rate detection of organoids Calcein staining method Appendix F (Normative) STR identification of organoids References