T/MMSP 11-2022 The determination method of aflatoxin B1 in honey using ELISA, an enzyme-linked immunosorbent assay screening method
T/MMSP 11-2022 The determination method of aflatoxin B1 in honey using ELISA, an enzyme-linked immunosorbent assay screening method
Basic Information
Scope
Scope: This document specifies the reagents and materials, instruments, sample preparation and storage, analysis steps, result determination, confirmatory methods, and other requirements for the ELISA enzyme-linked immunosorbent assay detection of aflatoxin B1 in honey. This document is applicable to the rapid detection of aflatoxin B1 in honey products during production and sales. Main technical content: Principle: The residual aflatoxin B1 in the sample competes with the aflatoxin B1 enzyme marker in the kit for the aflatoxin B1 antibody, forming an enzyme marker-antigen-antibody complex that reacts with the colorimetric reagent. The absorbance is measured with an enzyme analyzer, and the content of aflatoxin B1 in the sample is determined based on the absorbance value.