T/CHBAS 14-2021 Active Group standards

T/CHBAS 14-2021 Determination of Amino Acid Content in Milk Powder by High Performance Liquid Chromatography (HPLC) method

Publish Date: 2021-12-26 Implement Date: 2021-12-26 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CHBAS 14-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Specialty foods

ICS Name: Milk and processed milk products

Publish Date: 2021-12-26

Implement Date: 2021-12-26

Scope

Scope: This document specifies the principles, reagents and materials, instruments and equipment, experimental procedures, precision and detection limits for the determination of amino acids in milk powder by high-performance liquid chromatography (HPLC). This document is applicable to the determination of 17 amino acids in milk powder, including aspartic acid (Asp), glutamic acid (Glu), histidine (His), serine (Ser), arginine (Arg), glycine (Gly), threonine (Thr), proline (Pro), alanine (Ala), valine (Val), methionine (Met), cysteine (Cys), isoleucine (Ile), leucine (Leu), phenylalanine (Phe), lysine (Lys), and tyrosine (Tyr). Main technical content: The proteins in milk powder are hydrolyzed into free amino acids by hydrochloric acid, then subjected to the 2,4-dinitrochlorobenzene derivatization reaction. The amino acids are separated using a C18 chromatographic column and detected by an ultraviolet detector. Quantification is performed using an external standard method.