T/XEEPIA 001-2024 Determination of 16 sulfonamide antibiotics in water using solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry

Scope

Main technical content: This document specifies the method for determining 16 sulfanilamide antibiotics in water quality by solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry. This document is applicable to the determination of sulfanilamide acetate, sulfanilamide diacetate, sulfanilamide thiazole, sulfanilamide pyridine, sulfanilamide methyl dione, sulfanilamide methyl dione, trimethoprim, sulfanilamide dimethyl dione, sulfanilamide methoxydiazine, sulfanilamide methoxydiazine, sulfanilamide methoxydiazine, sulfanilamide oximethoxydiazine, sulfanilamide isoxazole, sulfanilamide phenylacetate, sulfanilamide dimethoxydione, and other 16 sulfanilamide antibiotics in surface water and urban wastewater treatment plant effluents. After sample filtration, the samples are enriched and purified by a solid-phase extraction column and then injected for separation and detection of sulfanilamide antibiotics by solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry. The sulfanilamide antibiotics are identified based on their retention times and characteristic ions, and quantified using an internal standard method. When the sample volume is 1.0 L, the container volume is 1.0 mL, and the injection volume is 5 μL, the method's detection limit is 0.001 μg/L to 0.462 μg/L, and the determination limit is 0.003 μg/L to 1.540 μg/L.

Development Information

Related Standards