T/CSBM 0026-2022 Method for Testing the Extracellular Mineralization of Biologically Active Glasses

Scope

Main Technical Content: 1. **Reagents and Testing Equipment** 1.1 **Reagents for Testing** - a) NaCl (analytical grade) - b) NaHCO3 (analytical grade) - c) KCl (analytical grade) - d) KHPO4·3H2O (analytical grade) - e) MgCl2·6H2O (analytical grade) - f) HCl solution (analytical grade) with a concentration of 1 mol/L - g) CaCl2 (analytical grade) - h) Na2SO4 (analytical grade) - i) Tris (analytical grade) - j) Deionized water 1.2 **Testing Equipment** - a) Heat-exchange magnetic stirrer - b) pH meter - c) Electronic analytical balance with an accuracy of 0.001 g - d) 2L beaker - e) 50 mL and 1 L graduated cylinders - f) 1 L volumetric flask - g) 1 L polytetrafluoroethylene (PTFE) bottle 2. **Preparation of Two Common In Vitro Mineralization Solutions** 2.1 **Tris-HCl Buffered Solution** - 2.1.1 **Reagents Required** - Deionized water: 700 mL - Tris: (6.00 ± 0.50) g - HCl solution: 35 mL with a concentration of 1 mol/L - 2.1.2 **Preparation Steps** - Under stirring, add 35 mL of 1 mol/L HCl solution to 700 mL deionized water. - Slowly add Tris until the pH does not exceed 7.45, and adjust the pH to 7.40. - Dilute the adjusted solution to 1000 mL and transfer it to a clean, smooth-surfaced PTFE bottle stored at 4°C for future use. **Note**: When using this solution, check and ensure that the Tris-HCl solution is clear. If it is cloudy, it cannot be used anymore and must be prepared anew. The solution has a shelf life of 30 days. 2.2 **Simulated Body Fluid** - 2.2.1 **Reagents and Quantities Required** See Table 1 for the reagents and quantities required to prepare simulated body fluid. **Table 1: Reagents and Quantities Required for Preparing Simulated Body Fluid** **Addition Order** **Reagent** **Addition Quantity** **1** **NaCl** **8.035 g** **

Development Information

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