T/SZGIA 2-2016 Environmental Microbial Detection Based on High-throughput Sequencing Part 2: Human Fecal Microbial Metagenomic Detection Method

Scope

Scope: This part is the second part of "Environmental Microbial Detection Based on High-Throughput Sequencing", which specifies the method of human fecal microbial metagenome detection using high-throughput sequencing technology, including sample collection, storage, laboratory procedures, sample processing procedures, and quality control, as well as sequencing data quality control. This part is applicable to human fecal microbial detection using metagenomics methods; Main technical content: This standard specifies the detection objects, detection requirements, sample collection, storage and transportation requirements; the detection steps include the conditions that the laboratory must meet; as well as the quality determination standards for DNA; library construction and high-throughput sequencing processes; and the requirements for data analysis and data quality control. Among them, the data quality control stipulates quality requirements and quantity requirements. After the sequencing is completed, the effective and available data quality value corresponding to each DNA sample should meet the following requirements: Q20≥90%, that is, more than 90% of the base quality values in the sequencing results of this sample are greater than 20; Q30≥80%, that is, more than 80% of the base quality values in the sequencing results of this sample are greater than 30. As for the quantity requirements: In order to complete subsequent data analysis steps such as species annotation and functional analysis, it is required that the number of short sequences sequenced for each sample be greater than 1 million.

Development Information

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