T/SHSYCXH 7-2022 The detection of the peripheral blood mononuclear cell population by flow cytometry
T/SHSYCXH 7-2022 The detection of the peripheral blood mononuclear cell population by flow cytometry
Basic Information
Scope
Scope: This document describes the method of using flow cytometry to detect the proportion of monocyte subpopulations in human peripheral blood, including the detection principle, reagents and consumables, instrumentation, pre-processing and analysis parameter settings, analysis steps, result calculation, and method characteristics, etc. This document is applicable to the identification and proportion determination of monocyte populations in human peripheral blood. Main technical content: 1. Scope 2. Normative reference documents 3. Terms and definitions 4. Abbreviations 5. Detection principle 6. Reagents and consumables 7. Instrumentation 8. Pre-processing and analysis parameter settings 9. Analysis steps 10. Result calculation 11. Method characteristics Appendix A (informative) - Schematic diagram of sample detection and analysis Appendix B (informative) - Example of minimum sampling quantity Appendix C (informative) - Example of repeatability