T/SHSYCXH 11-2022 Detection of free amino acids in serum by liquid chromatography tandem mass spectrometry
T/SHSYCXH 11-2022 Detection of free amino acids in serum by liquid chromatography tandem mass spectrometry
Basic Information
Scope
Scope: This document describes the method for determining the content of free amino acids in serum using liquid chromatography-tandem mass spectrometry, including the detection principle, the types of reagents, consumables, and instruments used, sample processing, instrument conditions, data processing, and precision. This document is applicable to the determination of the content of 17 free amino acids in serum listed in Appendix A; Main technical content: 1. Scope 2. Normative referenced documents 3. Terms and definitions 4. Detection principle 5. Reagents and consumables 6. Instrumentation and equipment 7. Test procedures 8. Result calculation 9. Precision Appendix A (informative) - Types of amino acids that can be measured and related information Appendix B (informative) - Reference conditions for liquid chromatography-tandem mass spectrometry parameters Appendix C (informative) - Chromatograms of mixed amino acid standard working solutions