T/GZCX 019-2022 Active Group standards

T/GZCX 019-2022 Determination of Polysaccharide Content in Shrubbery Rocket and Its Products

Publish Date: 2022-11-22 Implement Date: 2022-12-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GZCX 019-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Fruits and their products

Publish Date: 2022-11-22

Implement Date: 2022-12-01

Scope

Scope: This standard specifies the ultraviolet spectrophotometric method for determining the polysaccharide content in prickly pear and its products. This standard is applicable to the determination of polysaccharide content in fresh prickly pear, dried prickly pear, prickly pear preserved fruits, freeze-dried prickly pear powder, spray-dried prickly pear powder, prickly pear juice, and concentrated prickly pear juice, among other prickly pear and its products; Main technical content: 4. Method principle: Under the action of concentrated sulfuric acid, polysaccharides are hydrolyzed into monosaccharides and rapidly dehydrated to form aldehyde derivatives. The aldehyde derivatives react with phenol to form an orange-yellow solution, which exhibits a characteristic absorption at 490 nm. 5. Reagents and materials 5.1 Reagents: Except where otherwise specified, only analytical-grade reagents and first-grade water specified in GB/T 6682 shall be used. 5.1.1 Glucose (C6H12O6, CAS No.: 50-99-7) standard, with a purity of ≥ 98.0%. Before use, it should be dried at 105 °C until constant weight. 5.1.2 Sulfuric acid (H2SO4), ρ = 1.84 g/mL. 5.1.3 Ethanol (C2H6O). 5.1.4 Phenol (C6H6O), re-distilled. 5.2 Preparation of reagents 5.2.1 Glucose control solution (100 mg/L): Weigh 0.1 g (accurate to 0.001 g) of glucose standard, place it in a 100 mL beaker, dissolve it with a small amount of water, transfer it to a 1000 mL brown volumetric flask, and add water to the mark. Shake well and store it in a 4 °C refrigerator away from light. 5.2.2 80% ethanol solution: Measure 80 mL of anhydrous ethanol and place it in a 100 mL volumetric flask. Add water to the mark. 5.2.3 80% phenol solution: Weigh 80 g of phenol (accurate to 0.01 g), place it in a 100 mL beaker, dissolve it with a small amount of water, transfer it to a 100 mL brown volumetric flask, and add water to the mark. Shake well and store it in a 4 °C refrigerator away from light. 5.2.4 5% phenol solution: