T/PSC 1.7-2022 Ship ballast water inspection method Part 7: Total bacterial count
T/PSC 1.7-2022 Ship ballast water inspection method Part 7: Total bacterial count
Basic Information
Scope
Scope: This document specifies the methodological principles, reagents and materials, instruments and equipment, sample testing, data calculation and recording, and quality control for detecting the total number of bacteria in ship ballast water using fluorescence microscopy counting method. This document is applicable to the detection of the total number of bacteria in ship ballast water by fluorescence microscopy counting method; Main technical content: This standard utilizes the principle that bacteria, after being stained with DAPI and filtered by a black nuclear pore membrane, are retained on the membrane and can be observed under a fluorescence microscope, where the bacteria emit fluorescence. The total number of bacteria in the ballast water is detected and counted using the direct counting method of fluorescence microscopy, including sample collection, sample sampling, and the detection of the total number of bacteria in water samples, among other main technical contents.