T/PSC 1.2-2022 Ship ballast water monitoring method - Part 2: Living organisms larger than or equal to 10 μm and smaller than 50 μm

Scope

Scope: This document specifies the instruments, reagents, sample testing, data recording and calculation, quality assurance and control for detecting living organisms larger than or equal to 10 μm and smaller than 50 μm in ship ballast water samples using the dual fluorescence staining method. This document is applicable to the detection of living organisms larger than or equal to 10 μm and smaller than 50 μm in ship ballast water samples; Main technical content: This standard uses the fluorescent dyes fluorescein diacetate (FDA) and 5-chloromethylfluorescein diacetate (CMFDA) to co-stain the surviving organisms larger than or equal to 10 μm and smaller than 50 μm in ballast water, observe the viability of the organisms under a fluorescence microscope, and calculate the number of surviving organisms larger than or equal to 10 μm and smaller than 50 μm in the samples. The main technical content includes sample collection, sample testing, data recording and calculation, quality assurance and control, etc.

Development Information

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