T/PSC 1.4-2022 Ship ballast water inspection method - Part 4: Enterococci bacteria
T/PSC 1.4-2022 Ship ballast water inspection method - Part 4: Enterococci bacteria
Basic Information
Scope
Scope: This document specifies the methodological principles, reagents and materials, instruments and equipment, sample collection, sample testing, result calculation and presentation, quality control and assurance, and waste disposal for the determination of Enterococcus in ship ballast water by the enzyme substrate method. This document is applicable to the detection of Enterococcus in ship ballast water by the enzyme substrate method. Main technical content: This standard utilizes the principle that Enterococcus reacts with selective culture medium under specific temperature conditions for a specified period of time, resulting in a characteristic fluorescence phenomenon. By counting the number of positive samples with characteristic fluorescence and referring to the lookup table of 97-well quantitative plate results, the concentration value of Enterococcus in the sample can be calculated. The main technical content includes sample collection, sample testing, result calculation and presentation, quality assurance and control, and waste disposal