T/TPPA 0002-2022 RBL-2H3 mast cell RTCA method for evaluating allergy reactions of traditional Chinese medicine injection operation procedures
T/TPPA 0002-2022 RBL-2H3 mast cell RTCA method for evaluating allergy reactions of traditional Chinese medicine injection operation procedures
Basic Information
Scope
Main technical content: This standard specifies the method of evaluating allergic reactions to traditional Chinese medicine injections using RBL-2H3 mast cells RTCA, and provides a detailed description of the corresponding method. This standard includes the reagents, instruments, cells, cell culture solutions, and other materials required for the experiment, as well as the preparation of reagents and other operational steps. The research content of this standard includes the detection principle of the method, cell culture, subculture, cryopreservation and resuscitation, and the specific operation steps of RTCA detection. Statistical analysis software is used to analyze the experimental data, and the experimental data are expressed as mean ± s. When comparing the experimental groups with the negative control group, P < 0.05 indicates a significant difference, indicating a potential risk of allergic reactions.