T/BPCT 002-2024 Active Group standards

T/BPCT 002-2024 Determination of protease activity in dairy and dairy products

Publish Date: 2024-10-18 Implement Date: 2024-10-21 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/BPCT 002-2024

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Food

ICS Name: Comprehensive information about milk and dairy products

Publish Date: 2024-10-18

Implement Date: 2024-10-21

Scope

Scope: This document specifies a spectrophotometric method for determining the protease activity in milk and dairy products. This document is applicable to milk and dairy products, such as raw milk, sterilized milk, processed milk, fermented milk, milk powder, and foods made primarily from dairy products, for the determination of protease activity; Main technical content: Under certain temperature and pH conditions, protease in milk and dairy products hydrolyzes casein substrates to produce phenolic amino acids, which are reduced by specific reagents under alkaline conditions. The resulting product is measured by a spectrophotometer at a wavelength of 742 nm to determine the absorbance of the solution. The enzyme activity is directly proportional to the absorbance, so the enzyme activity of the sample can be calculated. It specifically includes the preparation of reagents and standards, instruments and equipment, analysis steps, and the calculation and expression of results, etc.