T/PSC 1.3-2022 Method for the detection of ballast water - Part 3: Escherichia coli bacteria
T/PSC 1.3-2022 Method for the detection of ballast water - Part 3: Escherichia coli bacteria
Basic Information
Scope
Scope: This document specifies the enzymatic substrate method for determining Escherichia coli in ship ballast water. This document is applicable to the detection of Escherichia coli in ship ballast water; Main technical content: This standard utilizes the principle that Escherichia coli, after being cultured for a specific period of time at a specific temperature, reacts with a selective culture medium, resulting in a yellow and characteristic fluorescence phenomenon. By counting the number of positive phenomena with yellow and characteristic fluorescence, and referring to the lookup table of 97-well quantitative plates, the concentration value of Escherichia coli in the sample can be calculated. The main technical contents include sample collection, sample detection, result calculation and presentation, quality assurance and control, and waste disposal