T/PSC 1.5-2022 Ship ballast water inspection method Part 5: Vibrio cholerae
T/PSC 1.5-2022 Ship ballast water inspection method Part 5: Vibrio cholerae
Basic Information
Scope
Scope: This document specifies the methodological principles, reagents and materials, instruments and equipment, sample processing, strain identification, virulence gene detection, and detection result reporting for the detection of Vibrio cholerae in ship ballast water using the isolation and culture method. This document is applicable to the detection of Vibrio cholerae in ship ballast water using the isolation and culture method. Main technical content: This standard uses thiosulfate citrate bile salts sucrose agar medium and Vibrio chromogenic medium to isolate and purify Vibrio cholerae from water samples enriched with alkaline peptone water, and further detects the number of Vibrio cholerae in the samples by biochemical identification and molecular biology methods, including sample processing methods, strain identification, virulence gene detection, and detection result reporting, etc.