T/BPCT 003-2024 The determination of lipase activity in dairy and dairy products
T/BPCT 003-2024 The determination of lipase activity in dairy and dairy products
Basic Information
Scope
Scope: This document specifies the method for determining the lipase activity in milk and dairy products. This document is applicable to milk and dairy products, such as raw milk, sterilized milk, processed milk, fermented milk, milk powder, and milk and dairy products made primarily from dairy products. Main technical content: The lipase in the sample can hydrolyze p-nitrophenyl palmitate to p-nitrophenol. Under alkaline conditions, p-nitrophenol turns yellow, and the absorbance of the solution is measured at a wavelength of 397 nm using an enzyme analyzer. The lipase activity is proportional to the absorbance, so the lipase activity of the sample can be calculated. It specifically includes the preparation of reagents and standards, instruments and equipment, analysis steps, and the calculation and presentation of results, etc.